Turapov, Obolbek, Waddell, Simon J, Burke, Bernard, Glenn, Sarah, Sarybaeva, Asel A, Tudo, Griselda, Labesse, Gilles, Young, Danielle I, Young, Michael, Andrew, Peter W, Butcher, Philip D, Cohen-Gonsaud, Martin and Mukamolova, Galina V (2014) Oleoyl-coenzyme A regulates interaction of transcriptional regulator RaaS (Rv1219c) with DNA in mycobacteria. Journal of biological chemistry, 289 (36). pp. 25241-25249. ISSN 1083-351X

	PDF - Published Version Restricted to SRO admin only Download (2MB)
	PDF - Published Version Download (2MB)

Abstract

Regulator of Antimicrobial-Assisted Survival), encoded by Rv1219c in Mycobacterium tuberculosis and bcg_1279c in Mycobacterium bovis BCG, plays an important role in mycobacterial survival in prolonged stationary phase and during murine infection. Here we demonstrate that long chain acyl-CoA derivatives (oleoyl-CoA and to lesser extent palmitoyl-CoA) modulate RaaS binding to DNA and the expression of the genes downstream that encode ATP-dependent efflux pumps. Moreover, exogenously added oleic acid influenced RaaS-mediated mycobacterial improvement of survival and the expression of the RaaS regulon. Our data suggest that long chain acyl-CoA derivatives serve as biological indicators of the bacterial metabolic state. Dysregulation of efflux pumps can be used eliminate non-growing mycobacteria.

Item Type:	Article
Schools and Departments:	Brighton and Sussex Medical School > Global Health and Infection
Subjects:	Q Science > QR Microbiology Q Science > QR Microbiology > QR0075 Bacteria
Depositing User:	Simon Waddell
Date Deposited:	05 Aug 2014 09:55
Last Modified:	02 Jul 2019 18:30
URI:	http://sro.sussex.ac.uk/id/eprint/49466

View download statistics for this item

📧 Request an update