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Tpz1TPP1 SUMOylation reveals evolutionary conservation of SUMO-dependent Stn1 telomere association

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posted on 2023-06-15, 14:44 authored by Mansi Garg, Resham L Gurung, Sahar Mansoubi, Jubed O Ahmed, Anoushka Dave, Felicity Watts, Alessandro BianchiAlessandro Bianchi
Elongation of the telomeric overhang by telomerase is counteracted by synthesis of the complementary strand by the CST complex, CTC1(Cdc13)/Stn1/Ten1. Interaction of budding yeast Stn1 with overhang-binding Cdc13 is increased by Cdc13 SUMOylation. Human and fission yeast CST instead interact with overhang-binding TPP1/POT1. We show that the fission yeast TPP1 ortholog, Tpz1, is SUMOylated. Tpz1 SUMOylation restricts telomere elongation and promotes Stn1/Ten1 telomere association,and a SUMO-Tpz1 fusion protein has increased affinity for Stn1. Our data suggest that SUMO inhibits telomerase through stimulation of Stn1/Ten1 action by Tpz1, highlighting the evolutionary conservation of the regulation of CST function by SUMOylation.

Funding

Analysis of regulation telomerase action & telomore capping function by the telomeric complex (Fellow: Bianchi); G0129; MRC-MEDICAL RESEARCH COUNCIL; G0701428

History

Publication status

  • Published

File Version

  • Published version

Journal

EMBO Reports

ISSN

1469-3178

Publisher

EMBO PRess

Issue

8

Volume

15

Page range

871-877

Department affiliated with

  • Sussex Centre for Genome Damage Stability Publications

Notes

Keywords: CST; Stn1; telomeres; Tpz1; SUMO

Full text available

  • Yes

Peer reviewed?

  • Yes

Legacy Posted Date

2014-07-11

First Open Access (FOA) Date

2014-07-11

First Compliant Deposit (FCD) Date

2014-07-11

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