University of Sussex
Browse
1-s2.0-S2211124714001193-main.pdf (2.32 MB)

Protein phosphatase 1 recruitment by Rif1 regulates DNA replication origin firing by counteracting DDK activity

Download (2.32 MB)
journal contribution
posted on 2023-06-08, 17:02 authored by Anoushka Dave, Carol Cooley, Mansi Garg, Alessandro BianchiAlessandro Bianchi
The eukaryotic genome is replicated according to a strict temporal program. Here, Bianchi and colleagues find that Rif1, a master regulator of the DNA replication program in yeast and mammals, exerts its effect on DNA replication origins by recruiting protein phosphatase 1 (PP1) to chromosomes. Rif1/PP1 counteracts the positive action of the DDK kinase on the replicative kinase MCM. In a final twist, kinase action on Rif1 is proposed to eventually release PP1 and allow origin firing.

Funding

Analysis of regulation telomerase action & telomore capping function by the telomeric complex (Fellow: Bianchi); G0129; MRC-MEDICAL RESEARCH COUNCIL; G0701428

History

Publication status

  • Published

File Version

  • Published version

Journal

Cell Reports

ISSN

2211-1247

Publisher

Elsevier

Issue

1

Volume

7

Page range

53-61

Department affiliated with

  • Sussex Centre for Genome Damage Stability Publications

Full text available

  • Yes

Peer reviewed?

  • Yes

Legacy Posted Date

2014-06-30

First Open Access (FOA) Date

2014-06-30

First Compliant Deposit (FCD) Date

2014-06-30

Usage metrics

    University of Sussex (Publications)

    Categories

    No categories selected

    Licence

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC