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Spd2 assists Spd1 in modulation of RNR architecture but does not regulate deoxynucleotide pools

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posted on 2023-06-08, 17:05 authored by Rasmus Vejrup-Hansen, Oliver Fleck, Katrine Landvad, Ulrik Fahnøe, Sebastian S Broendum, Ann-Sofie Schreurs, Birthe B Kragelund, Antony CarrAntony Carr, Christian Holmberg, Olaf Nielsen
In yeasts, small intrinsically disordered proteins (IDPs) modulate ribonucleotide reductase (RNR) activity to ensure an optimal supply of dNTPs for DNA synthesis. The S. pombe Spd1 protein can directly inhibit the large RNR subunit (R1), import the small subunit (R2) into the nucleus and induce an architectural change in the R1-R2 holocomplex. Here, we report the characterization of Spd2, a protein with homology to Spd1. We show that Spd2 is a CRL4Cdt2 controlled IDP that functions together with Spd1 in the DNA damage response and in modulation of RNR architecture. However, Spd2 does not regulate dNTP pools and R2 nuclear import. Furthermore, deletion of spd2 only weakly suppresses the Rad3ATR checkpoint dependency of CRL4Cdt2 mutants. However, when we raised intracellular dNTP pools by inactivation of RNR feedback inhibition, deletion of spd2 could suppress the checkpoint dependency of CRL4Cdt2 mutant cells to the same extent as spd1. Collectively, these observations suggest that Spd1 on its own regulates dNTP pools, while it together with Spd2 modulates RNR architecture and sensitizes cells to DNA damage.

History

Publication status

  • Published

File Version

  • Accepted version

Journal

Journal of Cell Science

ISSN

0021-9533

Publisher

Company of Biologists

Volume

127

Page range

2460-2470

Department affiliated with

  • Sussex Centre for Genome Damage Stability Publications

Notes

Running title: spd2 in fission yeast

Full text available

  • Yes

Peer reviewed?

  • Yes

Legacy Posted Date

2014-04-28

First Open Access (FOA) Date

2014-04-28

First Compliant Deposit (FCD) Date

2014-04-28

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