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Wheeler, Vanessa C, Prodromou, Chrisostomos, Pearl, Laurence H, Williamson, Robert and Coutelle, Charles (1996) Synthesis of a modified gene encoding human ornithine transcarbamylase for expression in mammalian mitochondrial and universal translation systems: a novel approach towards correction of a genetic defect. Gene, 169 (2). pp. 251-255. ISSN 0378-1119
Full text not available from this repository.Abstract
The mitochondrial (MT) genome is a potential means of gene delivery to human cells for therapeutic expression. As a first step towards this, we have synthesized a gene coding for mature human ornithine transcarbamylase (OTC) by recursive PCR using 18 oligodeoxyribonucleotides, each 70-80 nucleotides in length, using codons which should allow translation in accordance with both mammalian mt and universal codon usage. Flanking mt DNA sequences were incorporated which are designed to facilitate site-specific cloning into the mt genome. Expression of this human gene in Escherichia coli leads to an immunoreactive OTC product of the correct size and N-terminal amino-acid sequence, but which forms inclusion bodies and lacks enzymatic activity.
| Item Type: | Article |
|---|---|
| Keywords: | Amino Acid Sequence Animals Base Sequence Cell Line *Gene Expression *Gene Transfer Techniques Genetic Diseases, Inborn Humans Mammals Mitochondria/metabolism Molecular Sequence Data Ornithine Carbamoyltransferase/*genetics Ornithine Carbamoyltransferase Deficiency Disease Protein Biosynthesis |
| Schools and Departments: | School of Life Sciences > Biochemistry |
| Subjects: | Q Science > QD Chemistry > QD0241 Organic chemistry > QD0415 Biochemistry T Technology > TP Chemical technology > TP0248.13 Biotechnology |
| Depositing User: | Chrisostomos Prodromou |
| Date Deposited: | 24 Feb 2015 15:08 |
| Last Modified: | 24 Feb 2015 15:08 |
| URI: | http://sro.sussex.ac.uk/id/eprint/44389 |