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DNA fragmentation based combinatorial approaches to soluble protein expression Part II: library expression, screening and scale-up
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posted on 2023-06-08, 14:43 authored by Renos Savva, Chrisostomos ProdromouChrisostomos Prodromou, Paul C DriscollIn this second of a two-part review encompassing random, combinatorial methods for soluble protein 'domain hunting', we focus upon the expression screening from DNA fragment libraries. Given a library of domain length-encoding DNA fragments assembled in expression vectors, it is necessary to devise reliable means to screen the sample DNA fragment population to find those that express stable, soluble target protein fragments, suitable for the required downstream aims. This review summarizes a variety of alternative strategies that have been employed to identify such stable truncates of full-length proteins. In addition, we review measures that can determine the quality of the expressed protein, the likely reliability of these measures, and the apparent extent of their application within the featured studies.
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Publication status
- Published
Journal
Drug Discovery TodayISSN
1359-6446Publisher
ElsevierExternal DOI
Issue
21-22Volume
12Page range
939-947Department affiliated with
- Biochemistry Publications
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- No
Peer reviewed?
- Yes
Legacy Posted Date
2015-02-25Usage metrics
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