Qri2/Nse4, a component of the essential Smc5/6 DNA repair complex

Hu, Bin, Liao, Chunyan, Millson, Stefan H, Mollapour, Mehdi, Prodromou, Chrisostomos, Pearl, Laurence H, Piper, Peter W and Panaretou, Barry (2005) Qri2/Nse4, a component of the essential Smc5/6 DNA repair complex. Molecular Microbiology, 55 (6). pp. 1735-1750. ISSN 0950-382X

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We demonstrate a role for Qri2 in the essential DNA repair function of the Smc5/6 complex in Saccharomyces cerevisiae. We generated temperature-sensitive (ts) mutants in QRI2 and characterized their properties. The mutants arrest after S phase and prior to mitosis. Furthermore, the arrest is dependant on the Rad24 checkpoint, and is also accompanied by phosphorylation of the Rad53 checkpoint effector kinase. The mutants also display genome instability and are sensitive to agents that damage DNA. Two-hybrid screens reveal a physical interaction between Qri2 and proteins that are non-Smc elements of the Smc5/6 DNA repair complex, which is why we propose the name NSE4 for the open reading frame previously known as QRI2. A key role for Nse4 in Smc5/6 function is likely, as overexpressing known subunits of the Smc5/6 complex suppresses nse4(ts) cell cycle arrest. The nse4(ts) growth arrest is non-lethal and unlike the catastrophic nuclear fragmentation phenotype of smc6(ts) mutants, the nucleus remains intact; replicative intermediates and sheared DNA are not detected. This could imply a role for Nse4 in maintenance of higher order chromosome structure.

Item Type: Article
Keywords: Amino Acid Sequence Cell Cycle Cell Cycle Proteins/physiology Cell Nucleus/physiology Chromosome Structures/physiology *DNA Repair Genes, Fungal Intracellular Signaling Peptides and Proteins Molecular Sequence Data Protein Binding Protein-Serine-Threonine Kinases/physiology Saccharomyces cerevisiae/*enzymology/genetics Saccharomyces cerevisiae Proteins/chemistry/*genetics/*physiology Sequence Alignment Sequence Homology, Amino Acid Temperature Two-Hybrid System Techniques
Schools and Departments: School of Life Sciences > Biochemistry
Subjects: Q Science > QD Chemistry > QD0241 Organic chemistry > QD0415 Biochemistry
Q Science > QH Natural history > QH0301 Biology > QH0426 Genetics
Depositing User: Chrisostomos Prodromou
Date Deposited: 25 Feb 2015 06:56
Last Modified: 25 Feb 2015 06:56
URI: http://sro.sussex.ac.uk/id/eprint/44338
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