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Qri2/Nse4, a component of the essential Smc5/6 DNA repair complex
journal contribution
posted on 2023-06-08, 14:42 authored by Bin Hu, Chunyan Liao, Stefan H Millson, Mehdi Mollapour, Chrisostomos ProdromouChrisostomos Prodromou, Laurence PearlLaurence Pearl, Peter W Piper, Barry PanaretouWe demonstrate a role for Qri2 in the essential DNA repair function of the Smc5/6 complex in Saccharomyces cerevisiae. We generated temperature-sensitive (ts) mutants in QRI2 and characterized their properties. The mutants arrest after S phase and prior to mitosis. Furthermore, the arrest is dependant on the Rad24 checkpoint, and is also accompanied by phosphorylation of the Rad53 checkpoint effector kinase. The mutants also display genome instability and are sensitive to agents that damage DNA. Two-hybrid screens reveal a physical interaction between Qri2 and proteins that are non-Smc elements of the Smc5/6 DNA repair complex, which is why we propose the name NSE4 for the open reading frame previously known as QRI2. A key role for Nse4 in Smc5/6 function is likely, as overexpressing known subunits of the Smc5/6 complex suppresses nse4(ts) cell cycle arrest. The nse4(ts) growth arrest is non-lethal and unlike the catastrophic nuclear fragmentation phenotype of smc6(ts) mutants, the nucleus remains intact; replicative intermediates and sheared DNA are not detected. This could imply a role for Nse4 in maintenance of higher order chromosome structure.
History
Publication status
- Published
Journal
Molecular MicrobiologyISSN
0950-382XPublisher
WileyExternal DOI
Issue
6Volume
55Page range
1735-1750Department affiliated with
- Biochemistry Publications
Full text available
- No
Peer reviewed?
- Yes
Legacy Posted Date
2015-02-25Usage metrics
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No categories selectedKeywords
Amino Acid SequenceCell CycleCell Cycle Proteins/physiologyCell Nucleus/physiologyChromosome Structures/physiology*DNA RepairGenesFungalIntracellular Signaling Peptides and ProteinsMolecular Sequence DataProtein BindingProtein-Serine-Threonine Kinases/physiologySaccharomyces cerevisiae/*enzymology/geneticsSaccharomyces cerevisiae Proteins/chemistry/*genetics/*physiologySequence AlignmentSequence HomologyAmino AcidTemperatureTwo-Hybrid System Techniques
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