Toll-dependent antimicrobial responses in Drosophila larval fat body require Spätzle secreted by haemocytes

Shia, Alice K H, Glittenberg, Marcus, Thompson, Gavin, Weber, Alexander N, Reichhart, Jean-Marc and Ligoxygakis, Petros (2009) Toll-dependent antimicrobial responses in Drosophila larval fat body require Spätzle secreted by haemocytes. Journal of Cell Science, 122 (24). pp. 4505-4515. ISSN 1477-9137

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In Drosophila, the humoral response characterised by the synthesis of antimicrobial peptides (AMPs) in the fat body (the equivalent of the mammalian liver) and the cellular response mediated by haemocytes (blood cells) engaged in phagocytosis represent two major reactions that counter pathogens. Although considerable analysis has permitted the elucidation of mechanisms pertaining to the two responses individually, the mechanism of their coordination has been unclear. To characterise the signals with which infection might be communicated between blood cells and fat body, we ablated circulating haemocytes and defined the parameters of AMP gene activation in larvae. We found that targeted ablation of blood cells influenced the levels of AMP gene expression in the fat body following both septic injury and oral infection. Expression of the AMP gene drosomycin (a Toll target) was blocked when expression of the Toll ligand Spätzle was knocked down in haemocytes. These results show that in larvae, integration of the two responses in a systemic reaction depend on the production of a cytokine (spz), a process that strongly parallels the mammalian immune response.

Item Type: Article
Schools and Departments: Brighton and Sussex Medical School > Clinical and Experimental Medicine
Subjects: Q Science
Q Science > QR Microbiology
R Medicine
R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology Including cancer and carcinogens
Depositing User: Tracey O'Gorman
Date Deposited: 02 Nov 2012 16:39
Last Modified: 14 May 2015 09:42
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