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Single electrode dynamic clamp with StdpC

journal contribution
posted on 2023-06-08, 12:35 authored by David Samu, Vincenzo Marra, Ildiko KemenesIldiko Kemenes, Michael CrossleyMichael Crossley, George KemenesGeorge Kemenes, Kevin StarasKevin Staras, Thomas NowotnyThomas Nowotny
Dynamic clamp is a powerful approach for electrophysiological investigations allowing researchers to introduce artificial electrical components into target neurons to simulate ionic conductances, chemical or electrotonic inputs or connections to other cells. Due to the rapidly changing and potentially large current injections during dynamic clamp, problematic voltage artifacts appear on the electrode used to inject dynamic clamp currents into a target neuron. Dynamic clamp experiments, therefore, typically use two separate electrodes in the same cell, one for recording membrane potential and one for injecting currents. The requirement for two independent electrodes has been a limiting factor for the use of dynamic clamp in applications where dual recordings of this kind are difficult or impossible to achieve. The recent development of an active electrode compensation (AEC) method has overcome some of these prior limitations, permitting artifact-free dynamic clamp experimentation with a single electrode. Here we describe an AEC method for the free dynamic clamp software StdpC. The AEC component of StdpC is the first such system implemented for the use of non-expert users and comes with a set of semi-automated configuration and calibration procedures that facilitate its use. We briefly introduce the AEC method and its implementation in StdpC and then validate it with an electronic model cell and in two different biological preparations.

History

Publication status

  • Published

Journal

Journal of Neuroscience Methods

ISSN

0165-0270

Publisher

Elsevier

Issue

1

Volume

211

Page range

11-21

Department affiliated with

  • Neuroscience Publications

Full text available

  • No

Peer reviewed?

  • Yes

Legacy Posted Date

2012-10-31

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