File(s) under permanent embargo
Translation initiation factors and active sites of protein synthesis co-localize at the leading edge of migrating fibroblasts
journal contribution
posted on 2023-06-08, 12:30 authored by Mark Willett, Michele Brocard, Alexandre Davide, Simon MorleyCell migration is a highly controlled essential cellular process, often dysregulated in tumour cells, dynamically controlled by the architecture of the cell. Studies involving cellular fractionation and microarray profiling have previously identified functionally distinct mRNA populations specific to cellular organelles and architectural compartments. However, the interaction between the translational machinery itself and cellular structures is relatively unexplored. To help understand the role for the compartmentalization and localized protein synthesis in cell migration, we have used scanning confocal microscopy, immunofluorescence and a novel ribopuromycylation method to visualize translating ribosomes. In the present study we show that eIFs (eukaryotic initiation factors) localize to the leading edge of migrating MRC5 fibroblasts in a process dependent on TGN (trans-Golgi network) to plasma membrane vesicle transport. We show that eIF4E and eIF4GI are associated with the Golgi apparatus and membrane microdomains, and that a proportion of these proteins co-localize to sites of active translation at the leading edge of migrating cells.
History
Publication status
- Published
File Version
- Published version
Journal
The Biochemical JournalISSN
1470-8728Publisher
Portland PressExternal DOI
Issue
1Volume
438Page range
217-227Department affiliated with
- Biochemistry Publications
Full text available
- No
Peer reviewed?
- Yes
Legacy Posted Date
2012-10-29First Compliant Deposit (FCD) Date
2017-03-07Usage metrics
Categories
No categories selectedKeywords
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC