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Glutathione half-cell reduction potential and a-tocopherol as viability markers during the prolonged storage of Suaeda maritima seeds
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posted on 2023-06-08, 11:34 authored by Charlotte E Seal, Rosa Zammit, Peter Scott, Tim Flowers, Ilse KrannerAntioxidants protect seeds from oxidative damage during storage, supporting the maintenance of seed viability and the ability to germinate post-storage. No data on antioxidants during long-term storage are available for the seeds of the halophyte Suaeda maritima. Therefore, changes in lipid-soluble antioxidants in the tocopherol family (a-, ?-, d-tocopherol), were investigated in seeds stored for up to 16 years at 4°C at a seed moisture content of 10–13%, as well as changes in the water-soluble antioxidant glutathione (GSH) and electrolyte leakage. Seed oil content was also measured and determined to be 22%. During the first 3 years of storage, seed viability remained high and the concentration of total tocopherol was stable. Thereafter, both seed viability and a-tocopherol concentration rapidly decreased and electrolyte leakage increased, while ?-tocopherol and d-tocopherol concentrations did not correlate with seed viability. Although the concentrations of neither GSH nor glutathione disulphide (GSSG) alone were correlated with seed viability, the glutathione half-cell reduction potential (EGSSG/2GSH) was strongly correlated with viability throughout storage and increased before the onset of viability loss. Hence, in this species EGSSG/2GSH appeared to be an ‘early warning’ system preceding viability loss while a-tocopherol concentration changed concomitantly with viability.
History
Publication status
- Published
Journal
Seed Science ResearchISSN
0960-2585Publisher
Cambridge University PressExternal DOI
Issue
1Volume
20Page range
47-53Department affiliated with
- Biology and Environmental Science Publications
Full text available
- No
Peer reviewed?
- Yes
Legacy Posted Date
2012-05-14Usage metrics
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