Harvey, Diana M R, Hall, J L and Flowers, T J (1976) The use of freeze-substitution in the preparation of plant tissue for ion localization studies. Journal of Microscopy, 107 (2). pp. 189-198. ISSN 0022-2720
Full text not available from this repository.Abstract
A method, utilizing freeze substitution, is described for the preparation of plant tissue for analytical electron microscopy. The fine structure of the cytoplasm was adequately preserved after freezing leaf tissue in 2-methylbutane at −170°C. Furthermore, following substitution in ether, losses of sodium and potassium from the tissue were less than 4% of the original ion content and the loss of chloride was less than 1%. The merits of the procedure as a means of tissue preparation for ion localization studies are discussed.
Item Type: | Article |
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Schools and Departments: | School of Life Sciences > Biology and Environmental Science |
Subjects: | Q Science > QK Botany > QK0710 Plant physiology |
Depositing User: | Catrina Hey |
Date Deposited: | 15 May 2012 11:08 |
Last Modified: | 30 Nov 2012 17:13 |
URI: | http://sro.sussex.ac.uk/id/eprint/39168 |