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Improvement of quantitation of biological X-ray microanalysis

journal contribution
posted on 2023-06-08, 11:33 authored by Diane M R Harvey, Tim Flowers, B Kent
Absolute measurements of elemental concentrations within thin biological samples are often made by reference to a series of standards which resemble the samples in chemical and physical properties and the linear relationship between (p-b)/c and concentration. This principle requires that the chemical and physical properties of the matrix remain constant throughout a series of standards with different elemental contents and throughout different regions of the samples. Some of the changes undergone by specimens during X-ray microanalysis, e.g. loss of elements or organic mass loss, are also influenced by the composition of the matrix. A simple empirical modification to the linear (p-b)/c versus concentration relationship is presented to account for some of these effects and therefore improve quantitation of analyses.

History

Publication status

  • Published

Journal

Journal Of Microscopy

ISSN

0022-2720

Publisher

Blackwell Publishing

Issue

APR

Volume

134

Page range

93-100

Department affiliated with

  • Evolution, Behaviour and Environment Publications

Notes

Times Cited: 16 Harvey, dmr flowers, tj kent, b

Full text available

  • No

Peer reviewed?

  • Yes

Legacy Posted Date

2012-05-16

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