Hajibagheri, M A, Flowers, T J, Collins, J C and Yeo, A R (1988) A Comparison of the Methods of X-ray Microanalysis, Compartmental Analysis and Longitudional Ion Profiles to Estimate Cytoplasmic Ion Concentrations in Two Maize Varieties. Journal of Experimental Botany, 39 (3). pp. 279-290. ISSN 0022-0957

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Abstract

The ion content of compartments within plant root cells has been studied by three different methods; flux analysis using radioactive isotopes, longitudinal ion profiles and X-ray microanalysis. The data provide estimates of the concentrations of K+ and CI− in the cytoplasm of roots of culture solution and salt grown maize by three independent methods.

In the cultivar LG11 grown in 50 mol m−3 NaCl X-ray microanalysis, compartmental analysis and longitudinal profiles yielded approximately agreeing values for cytoplasmic K+ of 90, 70 and 62 mol m−33 of tissue volume respectively. However, the methods disagreed on cytoplasmic Cl− where the value obtained by compartmental analysis was about four times that from X-ray microanalysis and longitudinal profiles. Possible reasons for this are discussed.

Item Type:	Article
Additional Information:	Times Cited: 32 Hajibagheri, ma flowers, tj collins, jc yeo, ar
Schools and Departments:	School of Life Sciences > Evolution, Behaviour and Environment
Subjects:	Q Science
Depositing User:	Catrina Hey
Date Deposited:	10 May 2012 08:47
Last Modified:	30 Nov 2012 17:12
URI:	http://sro.sussex.ac.uk/id/eprint/39140

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