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Measuring cellular migration with image processing
journal contribution
posted on 2023-06-08, 11:21 authored by Constantino C Reyes-Aldasoro, D Biram, G M Tozer, C KanthouAn image-processing algorithm for analysis of migration of vascular endothelial cells in culture is presented. The algorithm correctly detected the cellular regions on either side of an artificial 'wound' made by dragging a sterile pipette tip across the monolayer of cells (scratch wound assay). Frequency filtering and mathematical morphology were used to approximate the boundaries of cellular regions. This allowed measurement of the distance between the regions, and therefore the migration rates, regardless of the orientation of the wound and even in cases where the cells were sparse and not tightly packed.
History
Publication status
- Published
Journal
Electronics LettersISSN
0013-5194Publisher
Institute of Electrical and Electronics EngineersIssue
13Volume
44Page range
791-793Department affiliated with
- Engineering and Design Publications
Notes
328GE Times Cited:4 Cited References Count:13Full text available
- No
Peer reviewed?
- Yes
Legacy Posted Date
2012-04-24Usage metrics
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