Birtić, Simona, Colville, Louise, Pritchard, Hugh W, Pearce, Stephen R and Kranner, Ilse (2011) Mathematically combined half-cell reduction potentials of low-molecular-weight thiols as markers of seed ageing. Free Radical Research, 45 (9). pp. 1093-1102. ISSN 1071-5762

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Abstract

The half-cell reduction potential of the glutathione disulphide (GSSG) / glutathione (GSH) redox couple appears to correlate with cell viability and has been proposed to be a marker of seed viability and ageing. We investigated the relationship between seed viability and the individual half-cell reduction potentials (Eis) of four low-molecular11 weight (LMW) thiols in Lathyrus pratensis seeds subjected to artificial ageing: GSH, cysteine (Cys), cysteinyl-glycine (Cys-Gly) and -glutamyl-cysteine (-Glu-Cys). The standard redox potential of -Glu-Cys was previously unknown and was experimentally determined. The Eis were mathematically combined to define a LMW thiol-disulphide based redox environment (Ethiol-disulphide). Loss of seed viability correlated with a shift in Ethiol-disulphide towards more positive values, with a LD50 value of -0.90 0.093 mV M (mean SD). The mathematical definition of Ethiol-disulphide is envisaged as a step towards the definition of the overall cellular redox environment, which will need to include all known redox-couples

Item Type:	Article
Additional Information:	Work part of D.Phil Thesis of Simona Birtic (co-supervised Dr Cranner (Kew).
Schools and Departments:	School of Life Sciences > Biology and Environmental Science
Depositing User:	Stephen Pearce
Date Deposited:	25 Feb 2013 10:19
Last Modified:	25 Feb 2013 10:25
URI:	http://sro.sussex.ac.uk/id/eprint/37564

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