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Revision of the nomenclature for the Bacillus thuringiensis pesticidal crystal proteins

journal contribution
posted on 2023-06-08, 09:59 authored by Neil CrickmoreNeil Crickmore, J Baum, D Dean, J Feitelson, D Lereclus, E Schnepf, J Van Rie, D Zeigler
The crystal proteins of Bacillus thuringiensis have been extensively studied because of their pesticidal properties and their high natural levels of production. The increasingly rapid characterization of new crystal protein genes, triggered by an effort to discover proteins with new pesticidal properties, has resulted in a variety of sequences and activities that no longer,fit the original nomenclature system proposed tn 1989. Bacillus thuringiensis pesticidal crystal protein (Cly and Cyt) nomenclature was initially based on insecticidal activity for the primary ranking criterion. Many exceptions to this systematic arrangement have become apparent, however; making the nomenclature system inconsistent. Additionally, the original nomenclature, with foul activity-based primary ranks for 13 genes, did not anticipate the current 73 holotype sequences that form many more than the original four subgroups. A new nomenclature, based on hierarchical clustering using amino acid sequence identity, is proposed. Roman numeral have been exchanged for Arabic numerals in the primary rank (e.g., Cry1Aa) to better accommodate the large number of expected new sequences. In this proposal, 133 crystal proteins comprising 24 primary ranks are systematically arranged.

History

Publication status

  • Published

Journal

Microbiology and Molecular Biology Reviews

ISSN

1092-2172

Publisher

American Society for Microbiology

Issue

3

Volume

62

Page range

807-813

Department affiliated with

  • Biochemistry Publications

Full text available

  • No

Peer reviewed?

  • Yes

Legacy Posted Date

2012-02-06

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