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End-labeling and analysis of Spo11-oligonucleotide complexes in Saccharomyces cerevisiae

journal contribution
posted on 2023-06-08, 09:49 authored by Matt NealeMatt Neale, S Keeney
During meiosis Spo11 catalyzes the formation of DNA double-strand breaks, becoming covalently attached to the 5' ends on both sides of the break during this process. Spo11 is removed from the DSB by single-stranded endonucleolytic cleavage flanking the DSB, liberating a short-lived species consisting of Spo11 protein covalently linked to a short oligonucleotide. The method presented here details how to detect these Spo11-oligo complexes in extracts made from meiotic yeast cells.

History

Publication status

  • Published

Journal

Methods in Molecular Biology

ISSN

10643745

Publisher

Humana Press Inc.

Volume

557

Page range

183-195

Book title

Methods in Molecular Biology

Department affiliated with

  • Sussex Centre for Genome Damage Stability Publications

Notes

Source Type: Book series (ISBN: 978-193411566-4), Document Type: Article

Full text available

  • No

Peer reviewed?

  • Yes

Legacy Posted Date

2012-02-06

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