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Compelling EPR evidence that the alternative oxidase is a diiron carboxylate protein

journal contribution
posted on 2023-06-08, 09:37 authored by Anthony Moore, Jane E Carré, Charles Affourtit, Mary Albury, Paul G Crichton, Kiyoshi Kita, Peter Heathcote
The alternative oxidase is a respiratory chain protein found in plants, fungi and some parasites that still remains physically uncharacterised. In this report we present EPR evidence from parallel mode experiments which reveal signals at approximately g = 16 in both purified alternative oxidase protein (g = 16.9), isolated mitochondrial membranes (g = 16.1), and in trypanosomal AOX expressed in Escherichia coli membranes (g = 16.4). Such signals are indicative of a dicarboxylate diiron centre at the active site of the enzyme. To our knowledge these data represent the first EPR signals from AOX present in its native environment.

History

Publication status

  • Published

Journal

BBA - Biochimica et Biophysica Acta

ISSN

0006-3002

Publisher

Elsevier

Volume

1777

Page range

327-330

Pages

4.0

Department affiliated with

  • Biochemistry Publications

Full text available

  • No

Peer reviewed?

  • Yes

Legacy Posted Date

2012-02-06

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