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Accurate whole human genome sequencing using reversible terminator chemistry

journal contribution
posted on 2023-06-08, 09:07 authored by Mark Osborne, David R Bentley, Shankar Balasubramanian, Harold P Swerdlow, et al
DNA sequence information underpins genetic research, enabling discoveries of important biological or medical benefit. Sequencing projects have traditionally used long (400800 base pair) reads, but the existence of reference sequences for the human and many other genomes makes it possible to develop new, fast approaches to re-sequencing, whereby shorter reads are compared to a reference to identify intraspecies genetic variation. Here we report an approach that generates several billion bases of accurate nucleotide sequence per experiment at low cost. Single molecules of DNA are attached to a flat surface, amplified in situ and used as templates for synthetic sequencing with fluorescent reversible terminator deoxyribonucleotides. Images of the surface are analysed to generate high-quality sequence.We demonstrate application of this approach to human genome sequencing on flow-sorted X chromosomes and then scale the approach to determine the genome sequence of a male Yoruba from Ibadan, Nigeria. We build an accurate consensus sequence from.303 average depth of paired 35-base reads. We characterize four million single-nucleotide polymorphisms and four hundred thousand structural variants, many of which were previously unknown. Our approach is effective for accurate, rapid and economical whole-genome re-sequencing and many other biomedical applications.

History

Publication status

  • Published

Journal

Nature

ISSN

0028-0836

Publisher

Nature Publishing Group

Issue

721

Volume

456

Page range

53-59

Pages

7.0

Department affiliated with

  • Chemistry Publications

Notes

Note: correspondence should go to David R. Bentley. Correspondence and requests for materials should be addressed to D.R.B. (Email: dbentley@illumina.com).

Full text available

  • No

Peer reviewed?

  • Yes

Legacy Posted Date

2012-02-06

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