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The Epstein Barr virus lytic cycle activator Zta interacts with methylated ZRE in the promoter of host target gene egr1
journal contribution
posted on 2023-06-08, 08:33 authored by James Heather, Kirsty Flower, Samine Isaac, Alison SinclairActivation of the host gene egr1 is essential for the lytic replication of EpsteinBarr virus (EBV). egr1 is activated by Zta (BZLF1, ZEBRA). Zta interacts directly with DNA through a series of closely related Zta-response elements (ZREs). Here we dissect the mechanism used by Zta to interact with the egr1 promoter and identify a weak interaction with egr1ZRE that is dependent on the distal part of egr1ZRE. Furthermore, we demonstrate that the ability of Zta to interact with egr1ZRE is enhanced at least tenfold by methylation. The ability of Zta to transactivate a reporter construct driven by the egr1 promoter can be enhanced by methylation. As the ability of Zta to interact with a methylated ZRE in the EBV genome correlates with its ability to activate the expression of the endogenous viral gene BRLF1, this suggests that Zta may also have the capability to overturn epigenetic control of egr1.
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Publication status
- Published
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- Published version
Journal
Journal of General VirologyISSN
0022-1317Publisher
Society for General MicrobiologyExternal DOI
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6Volume
90Page range
1450-1454Pages
5.0Department affiliated with
- Biochemistry Publications
Full text available
- Yes
Peer reviewed?
- Yes
Legacy Posted Date
2012-02-06First Open Access (FOA) Date
2017-11-15First Compliant Deposit (FCD) Date
2017-11-15Usage metrics
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