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Representational difference analysis of cDNA identifies novel genes expressed following preconditioning of the heart

journal contribution
posted on 2023-06-08, 08:31 authored by M A C Fauchon, T J Pell, G F Baxter, D M Yellon, D S Latchman, M F Hubank, Lynne Mayne
Preconditioning of the myocardium rapidly induces a number of transcription factors, which are likely to be responsible for a cascade of transcriptional changes underlying the development of delayed adaptation. Identifying these changes provides insight into the molecular pathways elicited by sub-lethal ischaemia and the mechanism leading to delayed adaptation. Genes up-regulated in rabbit myocardium in vivo by ischaemic preconditioning following reperfusion for 2 h, 4 h and 6 h posttreatment were identified by representational difference analysis of cDNA (cDNA. RDA). The area of the left ventricle rendered ischaemic by preconditioning or the equivalent area of sham-treated animals was isolated and cDNA.RDA performed. Three novel genes and six genes with known function where identified, including the TGFß receptor interacting protein 1, the a isoform of the A subunit of PP2 and the cap binding protein NCBP1. To determine whether expression of these genes correlated with preconditioning per se, expression was measured in myocardium after both ischaemic as well as heat shock induced preconditioning following 2 h, 4 h, and 6 h reperfusion. These genes were induced in rabbit myocardium in vivo by both ischaemia and heat shock, consistent with a fundamental role in the development of delayed adaptation. The well described role of PP2 in modulating the mitogen-activated protein kinase pathway and promoting cell survival is consistent with our previous work, which identified the reperfusion injury salvage kinase pathway in mediating the protective effects of ischaemic preconditioning. Expression of Trip1 and Ncbp1 also implicates TGFß signalling pathways and RNA processing and transport in delayed adaptation to stress in the myocardium.

History

Publication status

  • Published

Journal

Experimental and Molecular Medicine

ISSN

1226-3613

Issue

4

Volume

37

Page range

311-322

Pages

12.0

Department affiliated with

  • Biochemistry Publications

Notes

It reported a novel subtractive hybridisation method (RDA) for defining gene expression changes in vivo in ischaemic heart and defining molecular pathways for ischaemic preconditioning. RDA is now a routine procedure.

Full text available

  • No

Peer reviewed?

  • Yes

Legacy Posted Date

2012-02-06

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