Palecek, Jan, Vidot, Susanne, Feng, Min, Doherty, Aidan J and Lehmann, Alan R (2006) The Smc5-Smc6 DNA Repair Complex: BRIDGING OF THE Smc5-Smc6 HEADS BY THE KLEISIN, Nse4,AND NON-KLEISIN SUBUNITS. Journal of Biological Chemistry, 281 (48). pp. 36952-36959. ISSN 0021-9258

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Abstract

Structural maintenance of chromosomes (SMC) proteins play fundamental roles in many aspects of chromosome organization and dynamics. The SMC complexes form unique structures with long coiled-coil arms folded at a hinge domain, so that the globular N- and C-terminal domains are brought together to form a "head." Within the Smc5-Smc6 complex, we previously identified two subcomplexes containing Smc6-Smc5-Nse2 and Nse1-Nse3-Nse4. A third subcomplex containing Nse5 and -6 has also been identified recently. We present evidence that Nse4 is the kleisin component of the complex, which bridges the heads of Smc5 and -6. The C-terminal part of Nse4 interacts with the head domain of Smc5, and structural predictions for Nse4 proteins suggest similar motifs that are shared within the kleisin family. Specific mutations within a predicted winged helix motif of Nse4 destroy the interaction with Smc5. We propose that Nse4 and its orthologs form the delta-kleisin subfamily. We further show that Nse3, as well as Nse5 and Nse6, also bridge the heads of Smc5 and -6. The Nse1-Nse3-Nse4 and Nse5-Nse6 subcomplexes bind to the Smc5-Smc6 heads domain at different sites.

Item Type:	Article
Schools and Departments:	School of Life Sciences > Sussex Centre for Genome Damage and Stability
Depositing User:	Susanne Vidot
Date Deposited:	06 Feb 2012 20:57
Last Modified:	03 Apr 2012 08:28
URI:	http://sro.sussex.ac.uk/id/eprint/28819

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