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Extrasynaptic vesicle recycling in mature hippocampal neurons

journal contribution
posted on 2023-06-08, 07:32 authored by Arjuna Ratnayaka, Vincenzo Marra, Tiago Branco, Kevin StarasKevin Staras
Fast neuronal signalling relies on highly-regulated vesicle fusion and recycling at specialized presynaptic terminals. Recently, examples of non-classical neurotransmission have also been reported, where fusion of vesicles can occur at sites remote from conventional synapses. This has potentially broad biological implications, but the underlying mechanisms are not well established. Here, in mature hippocampal neurons, we demonstrate that a complete vesicle recycling pathway can occur at discrete axonal sites and that extrasynaptic fusion is a robust feature of native tissue. We show that laterally mobile vesicle clusters trafficking between synaptic terminals become transiently stabilized by evoked action potentials and undergo complete but delayed Ca2+-dependent fusion along axons. This fusion is associated with dynamic actin accumulation and subsequently, vesicles can be locally recycled, reacidified and re-used. Immunofluorescence and ultrastructural work demonstrates that extrasynaptic fusion sites can have apposed postsynaptic specializations, suggesting that mobile vesicle recycling may underlie highly dynamic neuron-neuron communication.

History

Publication status

  • Published

Journal

Nature Communications

ISSN

2041-1723

Publisher

Nature Publishing Group

Issue

11

Volume

2

Page range

531a

Department affiliated with

  • Neuroscience Publications

Full text available

  • No

Peer reviewed?

  • Yes

Legacy Posted Date

2012-02-06

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