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Co-expression of the mosquitocidal toxins Cyt1Aa and Cry11Aa from Bacillus thuringiensis subsp israelensis in Asticcacaulis excentricus

journal contribution
posted on 2023-06-08, 07:20 authored by Dasheng Zheng, Norma Adriana Valdez-Cruz, Gemma Armengol, Chloe Sevrez, Jose Maurilio Munoz-Olaya, Zhiming Yuan, Sergio Orduz, Neil CrickmoreNeil Crickmore
The cyt1Aa gene from Bacillus thuringiensis subsp. israelensis (Bti), whose product synergizes other mosquitocidal toxins, and functions as a repressor of resistance developed by mosquitoes against Bacilli insecticides, was introduced into the aquatic Gram-negative bacterium Asticcacaulis excentricus alongside the cry11Aa gene. The genes were introduced as an operon, but although mRNA was detected for both genes, no Cyt1Aa toxin was detected. Both proteins were expressed using a construct in which a promoter was inserted upstream of each gene. Recombinant A. excentricus expressing both toxins was found to be approximately twice as toxic to third instar larvae of Culex quinquefasciatus as transformants expressing just Cry11Aa.

History

Publication status

  • Published

Journal

Current Microbiology

ISSN

0343-8651

Issue

1

Volume

54

Page range

58-62

Pages

5.0

Department affiliated with

  • Biochemistry Publications

Full text available

  • No

Peer reviewed?

  • Yes

Legacy Posted Date

2012-02-06

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