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Regulatory analysis of the mouse Fgf3 gene: control of embryonic expression patterns and dependence upon sonic hedgehog (Shh) signalling
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posted on 2023-06-08, 07:14 authored by Nicola Powles, Heather Marshall, Androulla Economou, Chin Chiang, Akira Murakami, Clive Dickson, Robb Krumlauf, Mark MaconochieFgf3 displays a dynamic and complex expression pattern during mouse embryogenesis. To address the molecular mechanisms underlying Fgf3 expression, we used a transgenic approach to assay genomic regions from the mouse Fgf3 gene for regulatory activity. We identified an enhancer that mediates major components of embryonic expression, governing expression in the midbrain, hindbrain, surface ectoderm, dorsal roots and dorsal root ganglia (DRG), proximal sensory ganglia, and the developing central nervous system (CNS). Deletional analysis of the enhancer further delimited this regulatory activity to a 5.7-kb fragment. We have also revealed sonic hedgehog (Shh) -dependent and Shh-independent aspects of Fgf3 expression through breeding the Fgf3 reporter transgene into Shh mutants. In the absence of Shh signalling, Fgf3 reporter expression is lost in the ventral CNS, DRG, and superior cervical nerves, whereas activation of reporter expression in cranial ganglion cells is Shh independent. Moreover, detailed re-examination of the Shh phenotype revealed that Shh signalling is required for the correct development/maturation of the DRG. Developmental Dynamics 230:44¿56, 2004. © 2004 Wiley-Liss, Inc.
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Publication status
- Published
Journal
Developmental DynamicsISSN
1058-8388External DOI
Issue
1Volume
230Page range
44-56Pages
13.0Department affiliated with
- Neuroscience Publications
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- No
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- Yes
Legacy Posted Date
2012-02-06Usage metrics
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