Armengol, Gemma, Guevara, Oscar Enrique, Orduz, Sergio and Crickmore, Neil (2005) Expression of the Bacillus thuringiensis mosquitocidal toxin Cry11Aa in the aquatic bacterium Asticcacaulis excentricus. Current Microbiology, 51 (6). pp. 430-433. ISSN 0343-8651

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Abstract

A mosquitocidal aquatic bacterium has been developed by introducing an operon containing the cry11Aa, and p20 genes from Bacillus thuringiensis subsp. israelensis (Bti) into the gram-negative aquatic bacterium Asticcacaulis excentricus. After transformation, the cry11Aa gene was successfully expressed in recombinant A. excentricus under the tac promoter, at the level of 0.04 pg/cell. The recombinant bacteria were toxic to Aedes aegypti larvae with an LC50 of 6.83 x 10(5) cells/mL. We believe that these bacteria may have potential as genetically engineered microorganisms for the control of mosquito larvae.

Item Type:	Article
Schools and Departments:	School of Life Sciences > Biochemistry
Depositing User:	Neil Crickmore
Date Deposited:	06 Feb 2012 20:39
Last Modified:	06 Jun 2019 09:07
URI:	http://sro.sussex.ac.uk/id/eprint/27296

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