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Overproduction, purification and preliminary X-ray diffraction analysis of YncE, an iron-regulated Sec-dependent periplasmic protein from Escherichia coli

journal contribution
posted on 2023-06-08, 07:04 authored by Aisha Baba-Dikwa, Darren Thompson, Nick J Spencer, Simon C Andrews, Kimberly A Watson
The yncE gene of Escherichia coli encodes a predicted periplasmic protein of unknown function. The gene is de-repressed under iron restriction through the action of the global iron regulator Fur. This suggests a role in iron acquisition, which is supported by the presence of the adjacent yncD gene encoding a potential TonB-dependent outer-membrane transporter. Here, the preliminary crystallographic structure of YncE is reported, revealing that it consists of a seven-bladed [beta]-propeller which resembles the corresponding domain of the `surface-layer protein' of Methanosarcina mazei. A full structure determination is under way in order to provide insight into the function of this protein.

History

Publication status

  • Published

Journal

Acta Crystallographica Section F: Structural Biology Communications

ISSN

2053-230X

Publisher

International Union of Crystallography

Issue

10

Volume

64

Page range

966-969

Pages

4.0

Department affiliated with

  • Biochemistry Publications

Full text available

  • No

Peer reviewed?

  • Yes

Legacy Posted Date

2012-02-06

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