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Overproduction, purification and preliminary X-ray diffraction analysis of YncE, an iron-regulated Sec-dependent periplasmic protein from Escherichia coli
journal contribution
posted on 2023-06-08, 07:04 authored by Aisha Baba-Dikwa, Darren Thompson, Nick J Spencer, Simon C Andrews, Kimberly A WatsonThe yncE gene of Escherichia coli encodes a predicted periplasmic protein of unknown function. The gene is de-repressed under iron restriction through the action of the global iron regulator Fur. This suggests a role in iron acquisition, which is supported by the presence of the adjacent yncD gene encoding a potential TonB-dependent outer-membrane transporter. Here, the preliminary crystallographic structure of YncE is reported, revealing that it consists of a seven-bladed [beta]-propeller which resembles the corresponding domain of the `surface-layer protein' of Methanosarcina mazei. A full structure determination is under way in order to provide insight into the function of this protein.
History
Publication status
- Published
Journal
Acta Crystallographica Section F: Structural Biology CommunicationsISSN
2053-230XPublisher
International Union of CrystallographyExternal DOI
Issue
10Volume
64Page range
966-969Pages
4.0Department affiliated with
- Biochemistry Publications
Full text available
- No
Peer reviewed?
- Yes
Legacy Posted Date
2012-02-06Usage metrics
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