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Analysis of the shortvein cis-regulatory region of the decapentaplegic gene of Drosophila melanogaster
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posted on 2023-06-08, 07:01 authored by Brian G Stultz, Robert P Ray, Deborah A HurshIn mammals, the Transforming Growth Factor-ß (TGF-ß) superfamily controls a variety of developmental processes. In Drosophila, by contrast, a single member of the superfamily, decapentaplegic (dpp) performs most TGF-ß developmental functions. The complexity of dpp functions is reflected in the complex cis-regulatory sequences that flank the gene. Dpp is divided into three regions: Hin, including the protein-coding exons; disk, including 3' cis-regulatory sequences; and shortvein (shv), including noncoding exons and 5' cis-regulatory sequences. We analyzed the cis-regulatory structure of the shortvein region using a nested series of rearrangement breakpoints and rescue constructs. We delimit the molecular regions responsible for three mutant phenotypes: larval lethality, wing venation defects, and head capsule defects. Multiple overlapping elements are responsible for larval lethality and wing venation defects. However, the area regulating head capsule formation is distinct, and resides 5' to these elements. We have demonstrated this by isolating and describing two novel dpp alleles, which affect only the adult head capsule. genesis 42:181¿192, 2005. © 2005 Wiley-Liss, Inc.
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Publication status
- Published
Journal
GenesisISSN
1526-954XExternal DOI
Issue
3Volume
42Page range
181-192Pages
12.0Department affiliated with
- Evolution, Behaviour and Environment Publications
Notes
Collaboration with Dr. Hursh, article describes a genetic and molecular dissection of the 5'-cis-regulatory sequences of the BMP decapentaplegic, and identifies a new element of controlling head capsule morphogenesis. Dr. Ray's contribution included the molecular characterization of Tegula, the shortvein inter se, and the SEM study on head capsule defects.Full text available
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Peer reviewed?
- Yes
Legacy Posted Date
2012-02-06Usage metrics
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