University of Sussex
Browse

File(s) not publicly available

The protein kinase CK2 facilitates repair of chromosomal DNA single-strand breaks

journal contribution
posted on 2023-06-08, 06:48 authored by Joanna I Loizou, Sherif F El-Khamisy, Anastasia Zlatanou, David J Moore, Douglas W Chan, Jun Qin, Stefania Sarno, Flavio Meggio, Lorenzo A Pinna, Keith CaldecottKeith Caldecott
CK2 was the first protein kinase identified and is required for the proliferation and survival of mammalian cells. Here, we have identified an unanticipated role for CK2. We show that this essential protein kinase phosphorylates the scaffold protein XRCC1 and thereby enables the assembly and activity of DNA single-strand break repair protein complexes in vitro and at sites of chromosomal breakage. Moreover, we show that inhibiting XRCC1 phosphorylation by mutation of the CK2 phosphorylation sites or preventing CK2 activity using a highly specific inhibitor ablates the rapid repair of cellular DNA single-strand breaks by XRCC1. These data identify a direct role for CK2 in the repair of chromosomal DNA strand breaks and in maintaining genetic integrity.

History

Publication status

  • Published

Journal

Cell

ISSN

0092-8674

Issue

1

Volume

117

Page range

17-28

Pages

12.0

Department affiliated with

  • Sussex Centre for Genome Damage Stability Publications

Notes

Work was conducted in my laboraotory, by my student (JL)- External authors provided some materials. Identified a novel role for casein kinase in DNA repair.

Full text available

  • No

Peer reviewed?

  • Yes

Legacy Posted Date

2012-02-06

Usage metrics

    University of Sussex (Publications)

    Categories

    No categories selected

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC