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Zinc-Responsive Regulation of Alternative Ribosomal Protein Genes in Streptomyces coelicolor Involves Zur and (Sigma) R

journal contribution
posted on 2023-06-08, 06:25 authored by Gillian A Owen, Ben Pascoe, Dimitris Kallifidas, Mark PagetMark Paget
Streptomyces coelicolor contains paralogous versions of seven ribosomal proteins (S14, S18, L28, L31, L32, L33, and L36), which differ in their potential to bind structural zinc. The paralogues are termed C+ or C¿ on the basis of the presence or absence of putative cysteine ligands. Here, mutational studies suggest that the C¿ version of L31 can functionally replace its C+ paralogue only when expressed at an artificially elevated level. We show that the level of expression of four transcriptional units encoding C¿ proteins is elevated under conditions of zinc deprivation. Zur controls the expression of three transcriptional units (including rpmG2, rpmE2, rpmB2, rpsN2, rpmF2, and possibly rpsR2). Zur also controls the expression of the znuACB operon, which is predicted to encode a high-affinity zinc transport system. Surprisingly, the zinc-responsive control of the rpmG3-rpmJ2 operon is dictated by R, a sigma factor that was previously shown to control the response to disulfide stress in S. coelicolor. The induction of R activity during zinc limitation establishes an important link between thiol-disulfide metabolism and zinc homeostasis.

History

Publication status

  • Published

Journal

Journal of Bacteriology

ISSN

0021-9193

Issue

11

Volume

189

Page range

4078-4086

Pages

9.0

Department affiliated with

  • Biochemistry Publications

Full text available

  • No

Peer reviewed?

  • Yes

Legacy Posted Date

2012-02-06

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