University of Sussex
Browse

File(s) not publicly available

A combinatorial method to enable detailed investigation of protein–protein interactions

journal contribution
posted on 2023-06-08, 06:21 authored by Kate Maclagan, Rita Tommasi, Emmanuelle Laurine, Chrisostomos ProdromouChrisostomos Prodromou, Paul C Driscoll, Laurence PearlLaurence Pearl, Stefanie Reich, Renos Savva
BACKGROUND: Successful structural investigations of protein-protein interactions can be facilitated by studying only the core interacting regions of the constituent proteins. However, attempting the discovery of stable core complexes using informed trial-and-error approaches can prove time and resource intensive. METHODS: We describe a valuable extension of combinatorial domain hunting (CDH), a technology for the timely elucidation of soluble protein truncations. The new method, CDH(2), enables empirical discovery of stable protein-protein core complexes. CDH(2) is demonstrated ab initio using a previously well-characterized Hsp90/Cdc37 complex. RESULTS: Without using a priori information, we demonstrate the isolation of stable protein-protein complexes, suitable for further analyses. DISCUSSION: This resource-efficient process can provide protein complexes for screening of compounds designed to modulate protein-protein interactions, thus facilitating novel drug discovery.

History

Publication status

  • Published

Journal

Future Medicinal Chemistry

ISSN

1756-8919

Publisher

Future Science

Issue

3

Volume

3

Page range

271-282

Department affiliated with

  • Sussex Centre for Genome Damage Stability Publications

Full text available

  • No

Peer reviewed?

  • Yes

Legacy Posted Date

2012-02-06

Usage metrics

    University of Sussex (Publications)

    Categories

    No categories selected

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC