Crichton, Paul G, Affourtit, Charles and Moore, Anthony L (2007) Identification of a mitochondrial alcohol dehydrogenase in Schizosaccharomyces pombe: new insights into energy metabolism. Biochemical Journal, 401 (2). pp. 459-464. ISSN 0264-6021

Full text not available from this repository.

Abstract

In the present study we have shown that mitochondria isolated from Schizosaccharomyces pombe exhibit antimycin A-sensitive oxygen uptake activity that is exclusively dependent on ethanol and is inhibited by trifluoroethanol, a potent inhibitor of ADH (alcohol dehydrogenase). Ethanol-dependent respiratory activity has, to our knowledge, not been reported in S. pombe mitochondria to date, which is surprising as it has been concluded previously that only one ADH gene, encoding a cytosolic enzyme, occurs in this yeast. Spectrophotometric enzyme assays reveal that ADH activity in isolated mitochondria is increased ~16-fold by Triton X-100, which demonstrates that the enzyme is located in the matrix. Using genetic knockouts, we show conclusively that the novel mitochondrial ADH is encoded by adh4 and, as such, is unrelated to ADH isoenzymes found in mitochondria of other yeasts. By performing a modular-kinetic analysis of mitochondrial electron transfer, we furthermore show how ethanol-dependent respiratory activity (which involves oxidation of matrix-located NADH) compares with that observed when succinate or externally added NADH are used as substrates. This analysis reveals distinct kinetic differences between substrates which fully explain the lack of respiratory control generally observed during ethanol oxidation in yeast mitochondria.

Item Type:	Article
Additional Information:	Both co-authors were ALM's students and work carried out in ALM's lab.
Schools and Departments:	School of Life Sciences > Biochemistry
Depositing User:	Anthony Moore
Date Deposited:	06 Feb 2012 20:17
Last Modified:	22 Mar 2012 11:54
URI:	http://sro.sussex.ac.uk/id/eprint/25238

📧 Request an update