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Berglund, Gunnar I, Carlsson, Gunilla H, Smith, Andrew T, Szoke, Hanna, Henriksen, Anette and Hajdu, Janos (2002) The catalytic pathway of horseradish peroxidase at high resolution. Nature, 417. pp. 463-368. ISSN 0028-0836
Full text not available from this repository.Abstract
A molecular description of oxygen and peroxide activation in biological systems is difficult, because electrons liberated during X-ray data collection reduce the active centres of redox enzymes catalysing these reactions(1-5). Here we describe an effective strategy to obtain crystal structures for high-valency redox intermediates and present a three-dimensional movie of the X-ray-driven catalytic reduction of a bound dioxygen species in horseradish peroxidase (HRP). We also describe separate experiments in which high-resolution structures could be obtained for all five oxidation states of HRP, showing such structures with preserved redox states for the first time.
| Item Type: | Article |
|---|---|
| Additional Information: | ATS contributed the recombinant HRP, spectral deconvolution expertise, co-drafted the manuscript and coordinated the funding application. First description of the x-ray structures of classical horseradish peroxidase in all five oxidation states, including the catalytically relevant Fe (IV) intermediates and first catalytic movie for dioxygen reduction, cited 157 times. |
| Schools and Departments: | School of Life Sciences > Biochemistry |
| Depositing User: | Andrew Trevor Smith |
| Date Deposited: | 06 Feb 2012 20:15 |
| Last Modified: | 30 Nov 2012 17:06 |
| URI: | http://sro.sussex.ac.uk/id/eprint/25027 |