Hsu, Shang-Te Danny, Varnai, Peter, Bugaut, Anthony, Reszka, Anthony P, Neidle, Stephen and Balasubramanian, Shankar (2009) A G-Rich Sequence within the c-kit Oncogene Promoter Forms a Parallel G-Quadruplex Having Asymmetric G-Tetrad Dynamics. Journal of the American Chemical Society, 131 (37). pp. 13399-13409. ISSN 0002-7863

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Abstract

Guanine-rich DNA sequences with the ability to form quadruplex structures are enriched in the promoter regions of protein-coding genes, particularly those of proto-oncogenes. G-quadruplexes are structurally polymorphic and their folding topologies can depend on the sample conditions. We report here on a structural study using solution state NMR spectroscopy of a second G-quadruplex-forming motif (c-kit2) that has been recently identified in the promoter region of the c-kit oncogene. In the presence of potassium ions, c-kit2 exists as an ensemble of structures that share the same parallel-stranded propeller-type conformations. Subtle differences in structural dynamics have been identified using hydrogen-deuterium exchange experiments by NMR spectroscopy, suggesting the coexistence of at least two structurally similar but dynamically distinct substates, which undergo slow interconversion on the NMR timescale.

Item Type:	Article
Schools and Departments:	School of Life Sciences > Chemistry
Depositing User:	Peter Varnai
Date Deposited:	06 Feb 2012 20:11
Last Modified:	02 May 2012 14:10
URI:	http://sro.sussex.ac.uk/id/eprint/24593

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