Jones, George H, Paget, Mark S B, Chamberlin, Leony and Buttner, Mark J (1997) Sigma-E is required for the production of the antibiotic actinomycin in Streptomyces antibioticus. Molecular Microbiology, 23 (1). pp. 169-178. ISSN 0950382X

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Abstract

The phsA gene encodes phenoxazinone synthase (PHS), which catalyses the penultimate step in the pathway for actinomycin biosynthesis in Streptomyces antibioticus. The phsA promoter strikingly resembles a putative Streptomyces s(E) cognate promoter, and purified Es(E) holoenzyme transcribed the phsA promoter in vitro. However, the phsA promoter was still active in an S. antibioticus sigE null mutant and the level of PHS activity was unaffected. Despite this, disruption of sigE blocked actinomycin production completely. The loss of actinomycin production correlated with a 10-fold decrease in the activity of actinomycin synthetase I, the enzyme which catalyses the activation of the precursor of the actinomycin chromophore.

Item Type:	Article
Schools and Departments:	School of Life Sciences > Biochemistry
Depositing User:	Mark Paget
Date Deposited:	06 Feb 2012 20:08
Last Modified:	22 Mar 2012 10:26
URI:	http://sro.sussex.ac.uk/id/eprint/24274

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