Puglisi, Andrea, Bianchi, Alessandro, Lemmens, Laure, Damay, Pascal and Shore, David (2008) Distinct roles for yeast Stn1 in telomere capping and telomerase inhibition. EMBO Journal, 27 (17). pp. 2328-2339. ISSN 0261-4189
Full text not available from this repository.Abstract
The budding yeast Cdc13, Stn1 and Ten1 (CST) proteins are proposed to function as an RPA-like complex at telomeres that protects ('caps') chromosome ends and regulates their elongation by telomerase. We show that Stn1 has a critical function in both processes through the deployment of two separable domains. The N terminus of Stn1 interacts with Ten1 and carries out its essential capping function. The C terminus of Stn1 binds both Cdc13 and Pol12, and we present genetic data indicating that the Stn1-Cdc13 interaction is required to limit continuous telomerase action. Stn1 telomere association, similar to that of Cdc13, peaks during S phase. Significantly, the magnitude of Stn1 telomere binding is independent of telomere TG tract length, suggesting that the negative effect of Stn1 on telomerase action might be regulated by a modification of CST activity or structure in cis at individual telomeres. Genetic analysis suggests that the Tell kinase exerts an effect in parallel with the Stn1 C terminus to counteract its inhibition of telomerase. These data provide new insights into the coordination of telomere capping and telomerase regulation.
Item Type: | Article |
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Schools and Departments: | School of Life Sciences > Sussex Centre for Genome Damage and Stability |
Subjects: | Q Science |
Related URLs: | |
Depositing User: | Alessandro Bianchi |
Date Deposited: | 06 Feb 2012 20:01 |
Last Modified: | 19 Jun 2013 08:10 |
URI: | http://sro.sussex.ac.uk/id/eprint/23589 |