A single unbranched S-phase DNA damage and replication fork blockage checkpoint pathway

Marchetti, Maria A, Kumar, Sanjay, Hartsuiker, Edgar, Maftahi, Mohamed, Carr, Antony M, Freyer, Greg A, Burhans, William C and Huberman, Joel A (2002) A single unbranched S-phase DNA damage and replication fork blockage checkpoint pathway. Proceedings of the National Academy of Sciences, 99 (11). pp. 7472-7477. ISSN 1091-6490

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The eukaryotic intra-S-phase checkpoint, which slows DNA synthesis in response to DNA damage, is poorly understood. Is DNA damage recognized directly, or indirectly through its effects on replication forks? Is the slowing of S phase in part because of competition between DNA synthesis and recombination/repair processes? The results of our genetic analyses of the intra-S-phase checkpoint in the fission yeast, Schizosaccharomyces pombe, suggest that the slowing of S phase depends weakly on the helicases Rqh1 and Srs2 but not on other recombination/repair pathways. The slowing of S phase depends strongly on the six checkpoint-Rad proteins, on Cds1, and on Rad4/Cut5 (similar to budding yeast Dpb11, which interacts with DNA polymerase ¿) but not on Rhp9 (similar to budding yeast Rad9, necessary for direct damage recognition). These results suggest that, in fission yeast, the signal activating the intra-S-phase checkpoint is generated only when replication forks encounter DNA damage.

Item Type: Article
Schools and Departments: School of Life Sciences > Sussex Centre for Genome Damage and Stability
Depositing User: Edgar Hartsuiker
Date Deposited: 06 Feb 2012 19:58
Last Modified: 30 Nov 2012 17:05
URI: http://sro.sussex.ac.uk/id/eprint/23310
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