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Suppression of nitric oxide (NO)-dependent behavior by double-stranded RNA-mediated silencing of a neuronal NO synthase gene
journal contribution
posted on 2023-06-08, 00:45 authored by Sergei Korneev, Ildiko KemenesIldiko Kemenes, Volko Straub, Kevin StarasKevin Staras, Elena I Korneeva, George KemenesGeorge Kemenes, Paul R Benjamin, Michael O’SheaWe have used double-stranded RNA (dsRNA)-mediated RNA interference (RNAi) to disrupt neuronal nitric oxide (NO) synthase (nNOS) gene function in the snail Lymnaea stagnalis and have detected a speci?c behavioral phenotype. The injection of whole animals with synthetic dsRNA molecules targeted to the nNOS-encoding mRNA reduces feeding behavior in vivo and ?ctive feeding in vitro and interferes with NO synthesis by the CNS. By showing that synthetic dsRNA targeted to the nNOS mRNA causes a signi?cant and long-lasting reduction in the levels of Lym-nNOS mRNA, we verify that speci?c RNAi has occurred. Importantly, our results establish that the expression of nNOS gene is essential for normal feeding behavior. They also show that dsRNA can be used in the investigation of functional gene expression in the context of whole animal behavior, regardless of the availability of targeted mutation technologies.
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Publication status
- Published
File Version
- Published version
Journal
Journal of NeuroscienceISSN
0270-6474Publisher
Society for NeurosciencePublisher URL
Issue
11Volume
22Page range
RC227Department affiliated with
- Neuroscience Publications
Full text available
- Yes
Peer reviewed?
- Yes
Legacy Posted Date
2012-02-06First Open Access (FOA) Date
2016-03-22First Compliant Deposit (FCD) Date
2016-08-17Usage metrics
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