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The epitope of the H-type 2 trisaccharide recognized by Erythrina corallodendron lectin. Evidence for both attractive polar and strong hydrophobic interactions for complex formation involving a lectin

journal contribution
posted on 2023-06-08, 00:13 authored by Raymond U Lemieux, Chang-Chun Ling, Nathan Sharon, Hansjorg Streicher
Erythrina corallodendron lectin (ECorL), originally regarded as specific for N-acetyllactosamine (LacNAc), shows preferential binding of the II-type 2 human blood group determinant (alpha -L-Fuc-(1-2)-beta -D-Gal-(1-4)-beta -D-GlcNAc-OH). In the present study, various deoxy- and mono-O-methyl derivatives of beta -H-type 2 methyl glycoside and beta -LacNAc methyl glycoside were employed in an enzyme-linked lectin assay to further examine the specificity requirements of ECorL and the contribution of the fucose moiety to the binding of the trisaccharide to the lectin. Based on the relative affinities of the ligands, the X-ray structure of the ECorL/ LacNAc complex, and the site-directed mutagenesis studies of the lectin, the epitope of the II-type 2 trisaccharide recognized by ECorL has been delineated. The fucose moiety is shown to provide a strong hydrophobic interaction at its 2-OH, most likely with Trp 135 of the lectin combining site, in addition to the polar interactions of the key hydroxyl groups at positions 3 and 4 of the galactose and the stacking of the latter residue on Phe131. Comparison of the epitopes of H-type-2-OMe recognized by three other legume lectins, from Galactia tenuiflora, Psophocarpus tetragonolobus, and Ulex europaeus, shows that all four are different, in spite of the high similarity between their tertiary structures. Our findings provide a striking illustration of how a single oligosaccharide can carry different messages for communication with different recognition molecules.

History

Publication status

  • Published

Journal

Israel Journal of Chemistry

ISSN

0021-2148

Publisher

Wiley-VCH Verlag Berlin

Issue

3-4

Volume

40

Page range

167-176

Department affiliated with

  • Chemistry Publications

Full text available

  • No

Peer reviewed?

  • Yes

Legacy Posted Date

2012-02-06

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