University of Sussex
Browse

File(s) not publicly available

Evidence that the extracytoplasmic function sigma factor s(E) is required for normal cell wall structure in Streptomyces coelicolor A3(2)

journal contribution
posted on 2023-06-08, 00:07 authored by Mark PagetMark Paget, Leony Chamberlin, Abdelmadjid Atrih, Simon J Foster, Mark J Buttner
The sigE gene of Streptomyces coelicolor A3(2) encodes an RNA polymerase sigma factor belonging to the extracytoplasmic function (ECF) subfamily. Constructed sigE deletion and disruption mutants were more sensitive than the parent to muramidases such as hen egg white lysozyme and to the CwlA amidase from Bacillus subtilis. This correlated with an altered muropeptide profile, as determined by reverse-phase high-performance liquid chromatography analysis of lytic digests of purified peptidoglycan. The sigE mutants required high levels of magnesium for normal growth and sporulation, overproducing the antibiotic actino-rhodin and forming crenellated colonies in its absence. Together, these data suggest that sigE is required for normal cell wall structure. The role of s(E) was further investigated by analyzing the expression of hrdD, which is partially sigE dependent. The hrdD gene, which encodes the s(HrdD) subunit of RNA polymerase, is transcribed from two promoters, hrdDp1 and hrdDp2, both similar to promoters recognized by other ECF sigma factors. The activities of hrdDp1 and hrdDp2 were reduced 20- and 3-fold, respectively, in sigE mutants, although only hrdDp1 was recognized by Es(E) in vitro. Growth on media deficient in magnesium caused the induction of both hrdD promoters in a sigE-dependent manner

History

Publication status

  • Published

Journal

Journal of Bacteriology

ISSN

00219193

Issue

1

Volume

181

Page range

204-211

Department affiliated with

  • Biochemistry Publications

Full text available

  • No

Peer reviewed?

  • Yes

Legacy Posted Date

2012-02-06

Usage metrics

    University of Sussex (Publications)

    Categories

    No categories selected

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC