Function of the ypt2 gene in the exocytic pathway of Schizosaccharomyces pombe

Craighead, M W, Bowden, S, Watson, R and Armstrong, J (1993) Function of the ypt2 gene in the exocytic pathway of Schizosaccharomyces pombe. Molecular Biology of the Cell, 4 (10). pp. 1069-1076. ISSN 10591524

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The ypt2 gene of the fission yeast Schizosaccharomyces pombe encodes a member of the ypt/rab family of small GTP-binding proteins, related in sequence to Sec4p of Saccharomyces cerevisiae but closer to mammalian rab8. We have introduced a mutation into the gene corresponding to a mutation identified in ypt1, in which a conserved valine residue was altered to asparagine. The mutated ypt2 gene was introduced into the S. pombe genome by gene replacement. The resulting strain was temperature-sensitive for growth. Normal growth was restored by introduction of a plasmid-borne wild-type ypt2 cDNA or by cDNA for rab8 but not by various other rab or ypt sequences. At restrictive temperature the mutant cells accumulated the secretory protein acid phosphatase in a form that appeared to be fully glycosylated and acquired a population of vesicles detectable by electron microscopy. Thus the ypt2 protein, and by inference rab8, appear to function in the last stage of the secretory pathway.

Item Type: Article
Schools and Departments: School of Life Sciences > Biochemistry
Depositing User: John Armstrong
Date Deposited: 06 Feb 2012 19:47
Last Modified: 21 Mar 2012 15:06
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