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Co-localization in replication foci and interaction of human Y-family members, DNA polymerase pol? and REVl protein

journal contribution
posted on 2023-06-07, 23:54 authored by Agnès Tissier, Patricia Kannouche, Marie-Pierre Reck, Alan LehmannAlan Lehmann, Robert P P Fuchs, Agnès Cordonnier
The progress of replicative DNA polymerases along the replication fork may be impeded by the presence of lesions in the genome. One way to circumvent such hurdles involves the recruitment of specialized DNA polymerases that perform limited incorporation of nucleotides in the vicinity of the damaged site. This process entails DNA polymerase switch between replicative and specialized DNA polymerases. Five eukaryotic proteins can carry out translesion synthesis (TLS) of damaged DNA in vitro, DNA polymerases ¿, ¿, ¿, and ¿, and REV1. To identify novel proteins that interact with hpol¿, we performed a yeast two-hybrid screen. In this paper, we show that hREV1 interacts with hpol¿ as well as with hpol¿ and poorly with hpol¿. Furthermore, cellular localization analysis demonstrates that hREV1 is present, with hpol¿ in replication factories at stalled replication forks and is tightly associated with nuclear structures. This hREV1 nuclear localization occurs independently of the presence of hpol¿. Taken together, our data suggest a central role for hREV1 as a scaffold that recruits DNA polymerases involved in TLS.

History

Publication status

  • Published

Journal

DNA Repair

ISSN

1568-7864

Publisher

Elsevier

Issue

11

Volume

3

Page range

1503-1514

Pages

12.0

Department affiliated with

  • Sussex Centre for Genome Damage Stability Publications

Full text available

  • No

Peer reviewed?

  • Yes

Legacy Posted Date

2012-02-06

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