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Kinetics of re-establishing H3K79 methylation marks in global human chromatin.
journal contribution
posted on 2023-06-07, 23:21 authored by Steve Sweet, M Li, PM Thomas, K.R. Durbin, N.L KelleherWe employ a stable isotope strategy wherein both histones and their methylations are labeled in synchronized human cells. This allows us to differentiate between old and new methylations on pre-existing versus newly synthesized histones. The strategy is implemented on K79 methylation in an isoform-specific manner for histones H3.1, H3.2, and H3.3. Although levels of H3.3K79 monomethylation are higher than that of H3.2/H3.1, the rate of establishing the K79 methylation is the same for all three isoforms. Surprisingly, we find that pre-existing "old" histones continue to be K79-monomethylated and -dimethylated at a rate equal to the newly synthesized histones. These observations imply that some degree of positional "scrambling" of K79 methylation occurs through the cell cycle.
History
Publication status
- Published
Journal
Journal of Biological ChemistryISSN
00219258Publisher
The American Society for Biochemistry and Molecular BiologyExternal DOI
Issue
43Volume
285Page range
32778-32786Department affiliated with
- Sussex Centre for Genome Damage Stability Publications
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- No
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- Yes
Legacy Posted Date
2012-02-06Usage metrics
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