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Phosphorylation of survivin at threonine 34 inhibits its mitotic function and enhances its cytoprotective activity

journal contribution
posted on 2023-06-07, 14:57 authored by Rachel M.A. Barrett, Toby P. Osborne, Sally P. Wheatley
Survivin is an essential chromosomal passenger protein required for mitotic progression. It is also an inhibitor of apoptosis and can prevent caspase-mediated cell death. In addition, survivin levels are elevated in cancer cells where its presence correlates with increased resistance to chemo- and radio-therapy, which makes it an attractive target for novel anti-cancer strategies. Interestingly, survivin is phosphorylated by the mitotic kinase, cdk1, and a non-phosphorylatable form, survivin(T34A), cannot inhibit apoptosis. Here we rigorously test the ability of survivin(T34A) and its corresponding phosphomimetic, survivin(T34E), to promote cell viability through survivin's dual roles. The effects of these mutations are diametrically opposed: survivin(T34A) accelerates cell proliferation and promotes apoptosis, whereas survivin(T34E) retards growth and promotes survival. Thus the phosphorylation status of survivin at T34 is pivotal to a cell's decision to live or die.

History

Publication status

  • Published

Journal

Cell Cycle

ISSN

1538-4101

Publisher

LANDES BIOSCIENCE, 1002 WEST AVENUE, 2ND FLOOR, AUSTIN, TX 78701 USA

Issue

2

Volume

8

Page range

278-283

Department affiliated with

  • Sussex Centre for Genome Damage Stability Publications

Notes

In endnotes Ref:GDSC270

Full text available

  • Yes

Peer reviewed?

  • Yes

Legacy Posted Date

2009-02-24

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