University of Sussex
Browse

File(s) not publicly available

Purification, crystallization and preliminary X-ray analysis of a complex of the BRCT domains of human 53BP1 bound to the p53 tumour suppressor

journal contribution
posted on 2023-06-07, 23:16 authored by D J Derbyshire, B P Basu, T Date, K Iwabuchi, Aidan DohertyAidan Doherty
A complex of the DNA-binding domain of the tumour suppressor p53 bound to the BRCT domains of the p53-binding protein (53BP1) has been prepared and purified. Single crystals have been obtained using the microbatch technique with polyethylene glycol 4 kDa and ammonium sulfate. Crystals diffract X-rays to beyond 2.3 A and belong to the space group P2(1)2(1)2(1). Several complete data sets have been collected from a number of crystals, each with different unit-cell parameters. Partial structures have been produced by successful placement of two copies of the p53 core region into the asymmetric unit. There is clear evidence for the binding protein and a complete structure determination is under way.

History

Publication status

  • Published

Journal

Acta Crystallographica Section D: Biological Crystallography

ISSN

0907-4449

Issue

2

Volume

58-10

Page range

1826-1829

Pages

4.0

Department affiliated with

  • Sussex Centre for Genome Damage Stability Publications

Full text available

  • No

Peer reviewed?

  • Yes

Legacy Posted Date

2012-02-06

Usage metrics

    University of Sussex (Publications)

    Categories

    No categories selected

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC