pEg7, a new xenopus protein required for mitotic chromosome condensation in egg extracts

Cubizolles, Fabien, Legagneux, Vincent, Le Guellec, René, Chartrain, Isabelle, Uzbekov, Rustem, Ford, Chris and Le Guellec, Katherine (1998) pEg7, a new xenopus protein required for mitotic chromosome condensation in egg extracts. Journal of Cell Biology, 143 (6). pp. 1437-1446. ISSN 0021-9525

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We have isolated a cDNA, Eg7, corresponding to a Xenopus maternal mRNA, which is polyadenylated in mature oocytes and deadenylated in early embryos. This maternal mRNA encodes a protein, pEg7, whose expression is strongly increased during oocyte maturation. The tissue and cell expression pattern of pEg7 indicates that this protein is only readily detected in cultured cells and germ cells. Immunolocalization in Xenopus cultured cells indicates that pEg7 concentrates onto chromosomes during mitosis. A similar localization of pEg7 is observed when sperm chromatin is allowed to form mitotic chromosomes in cytostatic factor-arrested egg extracts. Incubating these extracts with antibodies directed against two distinct parts of pEg7 provokes a strong inhibition of the condensation and resolution of mitotic chromosomes. Biochemical experiments show that pEg7 associates with Xenopus chromosome-associated polypeptides C and E, two components of the 13S condensin.

Item Type: Article
Schools and Departments: School of Life Sciences > Biology and Environmental Science
Depositing User: EPrints Services
Date Deposited: 06 Feb 2012 19:28
Last Modified: 20 Sep 2012 08:17
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