Purification of the plant alternative oxidase from Arum maculatum: measurement, stability and metal requirement

Affourtit, Charles and Moore, Anthony L (2004) Purification of the plant alternative oxidase from Arum maculatum: measurement, stability and metal requirement. BBA - Biochimica et Biophysica Acta, 1608 (2-3). pp. 181-189. ISSN 0006-3002

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We have purified plant alternative oxidase (AOX) protein from the spadices of thermogenic Arum maculatum (cuckoo pint) to virtual homogeneity. The obtained enzyme fraction exhibits a high specific activity, consuming on average 32 µmol oxygen min-1 mg-1, which is completely stable for at least 6 months when the sample is stored at -70 °C. This exceptionally stable AOX activity is inhibited approximately 90% (I50not, vert, similar10 µM) by 8-hydroxyquinoline (8-OHQ) and also, although to a lesser extent, by other metal chelators such as o-phenanthroline, a,a'-dipyridyl and EDTA. When inhibited by 8-OHQ, AOX activity is fully restored upon addition of 1.2 mM ferric iron, but neither ferrous iron nor manganese has any effect, whilst zinc decreases activity even further. Furthermore, we have developed a spectrophotometric assay to measure AOX activity in an accurate manner, which will facilitate future steady state and transient kinetic studies. The reliability of this assay is evidenced by retained stability of AOX protein during the course of the reaction, reproducibility of the measured initial rates, an observed 2:1 duroquinol¿oxygen stoichiometry and by the fact that, in absolute terms, the measured rates of duroquinone formation and duroquinol disappearance are identical.

Item Type: Article
Additional Information: ALM is the PI on the grant, helped devise and conduct the expts. Describes a technique to purify the plant alternative oxidase which results in a stable protein product which is highly active and suitable for crystallisation studies.
Schools and Departments: School of Life Sciences > Biochemistry
Depositing User: Anthony Moore
Date Deposited: 06 Feb 2012 19:22
Last Modified: 11 Jun 2012 09:37
URI: http://sro.sussex.ac.uk/id/eprint/20327
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