University of Sussex
Browse

File(s) not publicly available

An emilin family extracellular matris protein identified inthe cochlear basilar membrane

journal contribution
posted on 2023-06-07, 22:42 authored by Lori L Amma, Richard GoodyearRichard Goodyear, Jonathan S Faris, Iwan Jones, Lily Ng, Guy Richardson, Douglas Forrest
The precise movement of the cochlear basilar membrane (BM) stimulates the sensory hair cells during auditory transduction. However, the molecular composition of the BM that confers its specialized properties of support and elasticity is poorly understood. A differential screen of cochlear RNA from deaf mice lacking thyroid hormone receptor ß was used to identify a sequence encoding a secreted protein, which is abundant in the BM and is expressed at low levels in the heart, lung, and brain. The protein possesses several domains for protein interactions and is related to emilin (elastin microfibril interface-located protein) previously isolated from aorta. This cochlear emilin-2 mRNA is expressed in the tympanic border cells underlying the BM and an antibody detected protein in the extracellular matrix surrounding the collagenous fibers in the BM. These results identify emilin-2 as a major BM component and suggest that it contributes to the developmental assembly or function of the BM.

History

Publication status

  • Published

Journal

Molecular and Cellular Neuroscience

ISSN

1044-7431

Issue

3

Volume

23

Page range

460-472

Department affiliated with

  • Neuroscience Publications

Full text available

  • No

Peer reviewed?

  • Yes

Legacy Posted Date

2012-02-06

Usage metrics

    University of Sussex (Publications)

    Categories

    No categories selected

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC