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Mitochondrial electron transfer in the wheat pathogenic fungus i Septoria tritici - on the role of alternative respiratory enzymes in fungicide resistance.

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posted on 2023-06-07, 22:37 authored by Charles Affourtit, Steve P Heaney, Anthony Moore
Certain phytopathogenic fungi are able to express alternative NADH- and quinol-oxidising enzymes that are insensitive to inhibitors of the mitochondrial respiratory Complexes I and III. To assess the extent to which such enzymes confer tolerance to respiration-targeted fungicides, an understanding of mitochondrial electron transfer in these species is required. An isolation procedure has been developed which results in intact, active and coupled mitochondria from the wheat pathogen Septoria tritici, as evidenced by morphological and kinetic data. Exogenous NADH, succinate and malate/glutamate are readily oxidised, the latter activity being only partly (approx. 70%) sensitive to rotenone. Of particular importance was the finding that azoxystrobin (a strobilurin fungicide) potently inhibits fungal respiration at the level of Complex III. In some S. tritici strains investigated, a small but significant part of the respiratory activity (approx. 10%) is insensitive to antimycin A and azoxystrobin. Such resistant activity is sensitive to octyl gallate, a specific inhibitor of the plant alternative oxidase, This enzyme, however, could not be detected immunologically. On the basis of the above findings, a conceptual mitochondrial electron transfer chain is presented. Data are discussed in terms of developmental and environmental regulation of the composition of this chain. (

History

Publication status

  • Published

ISSN

0005-2728

Publisher

ELSEVIER SCIENCE BV, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS

Issue

2-3

Volume

1459

Page range

291-298

Presentation Type

  • paper

Event name

11th European Bioenergetics Conference (EBEC)

Event location

UNIV SUSSEX, BRIGHTON, ENGLAND, SEP 09-14, 2000

Event type

conference

ISBN

0006-3002

Department affiliated with

  • Biochemistry Publications

Notes

Published in BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS

Full text available

  • No

Peer reviewed?

  • Yes

Legacy Posted Date

2012-02-06

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